Trace metals, such as mercury, lead, arsenic, and cadmium are cumulative toxicants in wildlife with important implications for both flora and fauna in freshwater systems. Breast feathers can be used to monitor the concentrations of trace metals in waterbirds, providing an accurate measure of body burdens and acting as bioindicators of pollution in freshwater systems where these waterbirds feed. Trace metal levels were studied in 26 feather samples from three different species of duck: Pacific black duck (Anas superciliosa), grey teal (Anas gracilis), and Australian wood duck (Chenonetta jubata) from Black Swamp (Black Dog Creek) Wildlife Reserve, northern Victoria, Australia. Breast feathers were sampled from ducks harvested by recreational hunters between 14th and 16th May 2020. Samples were cut and washed on an orbital shaker, using a solution of chloroform and methanol to remove external contamination. Samples were digested in concentrated nitric acid and analyzed using inductively coupled plasma-mass spectroscopy (ICP-MS). Overall, we found that the mean concentrations of all trace metals were higher in grey teal when compared to other ducks, with the exception of cadmium, which was highest in Australian wood ducks. Studies on trace metal concentrations in Australian waterbirds are limited, and this is the first study in Victorian ducks. This work provides an indication of the bioavailability of trace metals in Victorian freshwater systems.