Humans are exposed to microplastics (MPs) when eating and drinking [1]. The average person ingests 5 grams of MPs per week [2]. Seafood, a crucial part of the human diet, has become a target of interest to MP ingestion studies. However, MP extraction from complex tissues, such as the digestive organs in prawns, is challenging. The internal wall of the gastrointestinal tract (GIT) of decapods are often coated by complexes of chitin and protein in the fore and hind gut [3]. Traditional MP extraction protocols, like potassium hydroxide, perform well on protein or lipid-rich tissues but not for chitin-rich prawn GIT [4]. Microwave-assisted oxidant digestion methods have been applied to obtain chitosan and oligoglucosamine from crustacean exoskeletons [5][6]. This method shows promise in breaking down the prawn GIT tissue but the efficacy and impact on MP particles is not yet known. Ergo, this work investigates five digestion methods (acid, alkaline, enzyme, oxidant, microwave assisted oxidant reactions) for MP extraction from prawn GIT by comparing the digestion time, digestive efficiency, presence of undigested residuals, overall digestion level, and effect on reference polymers (PA, PE, PES, PP, PS, PVC, rayon) characteristics. The undigested residuals have been visualised using lactophenol cotton blue staining, and subsequently quantified using computer vision to assess the average blue value in the staining and the percent coverage area on the filters. Overall, the microwave-assisted oxidant method achieved the best performance by digesting the GIT in 10% hydrogen peroxide and microwaving the mixture for 10 minutes after boiling. The presence of undigested residuals had an average blue value of 151.81 and 10.13% filter coverage. A digestive efficiency of 96.85% and good overall digestion level was also achieved. The physical and chemical characteristics of the six types of reference polymers underwent little change after digestion except for rayon.